Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
karyopherin α1 Double Nickase Plasmid (h): sc-402324-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- karyopherin α1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- karyopherin α1 Double Nickase Plasmid (h) and karyopherin α1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting KPNA1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: karyopherin α1 Antibody (187.1): sc-101292
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
karyopherin α1 Double Nickase Plasmid (h) | sc-402324-NIC | 20 µg | $410.00 | |||
karyopherin α1 Double Nickase Plasmid (h2) | sc-402324-NIC-2 | 20 µg | $410.00 |
KPNA1 encodes karyopherin alpha 1 (importin-α5), an adaptor in the classical nuclear import pathway that recognizes basic nuclear localization signals on cargo proteins and couples them to importin-β for Ran GTPase–dependent translocation through the nuclear pore complex. By controlling nucleocytoplasmic trafficking, KPNA1 influences cell-cycle progression, DNA damage signaling, innate immune transcriptional responses, and stress-adaptive programs through regulated nuclear access of transcription factors and regulatory enzymes. Altered importin-mediated transport has been linked to dysregulated signaling networks and remodeling of gene expression states observed in cancer and neurodegenerative and inflammatory contexts. KPNA1 is also studied in host–pathogen interactions where nuclear import of viral or bacterial effectors can shape replication and immune evasion mechanisms.
karyopherin α1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the KPNA1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within KPNA1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt KPNA1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of KPNA1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.