Kanamycin A sulfate (CAS 25389-94-0)

Kanamycin A sulfate is an aminoglycoside antibiotic that functions by binding to the 30S subunit of bacterial ribosomes, thereby inhibiting protein synthesis. This binding interferes with the accurate reading of the genetic code during translation, leading to the production of faulty or incomplete proteins. As a result, the bacterial cells are unable to carry out essential cellular processes, ultimately leading to their death. Kanamycin A sulfate achieves this by causing misreading of the mRNA codons, leading to the incorporation of incorrect amino acids into the growing peptide chain. This disruption of protein synthesis ultimately disrupts the normal functioning of the bacterial cells, making them unable to survive and replicate. Additionally, Kanamycin A sulfate induces the production of reactive oxygen species within bacterial cells, further contributing to their demise. Overall, the mechanism of action of Kanamycin A sulfate involves targeting the bacterial ribosomes and interfering with protein synthesis, ultimately leading to the inhibition of essential cellular processes and the eventual death of the bacterial cells.