KRAS (h): 293T Lysate: sc-111225

K-Ras (h): 293 Lysate, derived from the HEK 293 cell line, is instrumental in studying the Kirsten rat sarcoma viral oncogene homolog (K-Ras) in human cells, focusing on its role in cellular signaling pathways that regulate cell division and growth. This lysate is crucial for investigating the biochemical mechanisms by which K-Ras mediates signal transduction from the cell membrane to the nucleus, influencing gene expression without clinical implications. The 293 cell line, known for its origin from human embryonic kidney cells, provides a robust system for the high-level expression and study of oncogenes like K-Ras. Researchers use this lysate to dissect the interaction of K-Ras with GTP/GDP binding and its role in the activation of downstream effectors such as RAF, MEK, and ERK, which are pivotal in controlling cellular proliferation and differentiation. Techniques such as pull-down assays, GTPase activity assays, and Western blotting are commonly employed to analyze these interactions. Through such studies, scientists can understand the fundamental aspects of Ras signaling pathways, exploring K-Ras's influence on cellular architecture and signaling dynamics.