K-cadherin Double Nickase Plasmid (h): sc-401455-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- K-cadherin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- K-cadherin Double Nickase Plasmid (h) and K-cadherin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CDH6. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: K-cadherin Antibody (2B6): sc-59974
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
K-cadherin Double Nickase Plasmid (h) | sc-401455-NIC | 20 µg | $410.00 | |||
K-cadherin Double Nickase Plasmid (h2) | sc-401455-NIC-2 | 20 µg | $410.00 |
CDH6 encodes K-cadherin, a classical calcium-dependent cell–cell adhesion molecule that supports epithelial cohesion and tissue morphogenesis through homophilic interactions at adherens junctions. By coupling to catenins and the actin cytoskeleton, K-cadherin contributes to contact-dependent signaling, cell polarity, and coordinated migration programs. CDH6 expression is prominent during development, including in renal and neural tissues, and its dysregulation has been associated with altered adhesion states linked to tumor progression and invasion-related phenotypes. These properties make CDH6 a useful target for studying cadherin-mediated junction dynamics, epithelial–mesenchymal transitions, and adhesion-dependent signaling networks.
K-cadherin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CDH6 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CDH6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CDH6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CDH6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.