K-562 + PMA nuclear extract: sc-2131
- supplied in four vials, each containing 250 µg nuclear extract in 50 µl buffer
- provided in 20 mM HEPES (pH 7.9), 20% v/v glycerol, 0.1 M KCI, 0.2 mM EDTA, 0.5 mM PMSF and 0.5 mM DTT
- human nuclear extract; chronic myelogenous leukemia cells induced with phorbol
- suitable for use in Gel Shift and Western Blotting assays
- Extracts should be stored at -70°C and repeated freezing and thawing should be avoided.
- prepared by the method of Dignam et al., (1983) Nucleic Acids Res. 11: 1475
QUICK LINKS
SEE ALSO...
K-562 + PMA nuclear extract integrates the K-562 cell line, derived from a chronic myeloid leukemia patient, with Phorbol 12-myristate 13-acetate (PMA), an activator of protein kinase C (PKC). This combination is utilized in research to explore the PKC-mediated signaling pathways within leukemic cells, focusing on transcriptional regulation and signal transduction. The addition of PMA to the K-562 nuclear extract enables researchers to specifically investigate how PKC activation affects the nuclear factors and gene expression profiles in leukemic cells. This nuclear extract is instrumental in conducting chromatin immunoprecipitation (ChIP) assays to delineate how activated PKC influences transcription factor binding to DNA. It is also used in electrophoretic mobility shift assays (EMSA) to assess the impact of PKC activation on the DNA-binding activities of nuclear proteins. Through these methodologies, K-562 + PMA nuclear extract helps delineate the role of PKC in altering gene expression and cellular behaviors in a fundamental research setting, providing valuable insights into the nuclear dynamics of leukemic cells. The origin of the K-562 cell line is meticulously verified, ensuring consistent and accurate experimental results in basic biological research applications.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
K-562 + PMA nuclear extract | sc-2131 | 250 µg/0.05 ml | $160.00 |