Jurkat + PMA nuclear extract: sc-2133

Jurkat + PMA nuclear extract is a specialized preparation derived from Jurkat cells, a human T lymphocyte cell line, treated with Phorbol 12-myristate 13-acetate (PMA), which is a potent activator of protein kinase C (PKC). This extract is extensively used in research to study the mechanisms of T-cell activation and the signaling pathways influenced by PKC. By adding PMA, researchers can mimic conditions that lead to T-cell activation, making it possible to explore how PKC influences various nuclear processes, including the transcription of genes involved in cell proliferation and immune response. The extract is particularly valuable in assays such as chromatin immunoprecipitation (ChIP) and electrophoretic mobility shift assays (EMSA), where it helps in identifying the specific binding sites of transcription factors and the impact of PKC activation on these interactions. Furthermore, this nuclear extract facilitates the study of modifications in chromatin structure under stimulated conditions, providing insights into the dynamics of chromatin remodeling in response to external signals. The utilization of Jurkat + PMA nuclear extract in these research settings highlights its role in advancing our understanding of T-cell biology, ensuring that findings are relevant to basic immunological processes. The origin and treatment of the Jurkat cells are meticulously verified to maintain consistency and reliability in experimental results.