Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Junctophilin-2 CRISPR/Cas9 KO Plasmid (h): sc-401805
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Junctophilin-2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Junctophilin-2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Junctophilin-2 Antibody (H-3): sc-377086
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Junctophilin-2 CRISPR/Cas9 KO Plasmid (h) | sc-401805 | 20 µg | $397.00 |
Overview
JPH2 encodes junctophilin-2, a membrane tethering protein that stabilizes junctional membrane complexes between the plasma membrane/T-tubules and sarcoplasmic reticulum in striated muscle. By maintaining nanoscale coupling between L-type Ca²⁺ channels and ryanodine receptors, JUNCTOPHILIN-2 supports excitation–contraction coupling and efficient intracellular Ca²⁺ release. JPH2 function intersects with Ca²⁺ handling pathways that influence contractility, electrical signaling, and downstream calcium-dependent transcriptional programs. Altered JPH2 expression or organization has been linked to cardiomyocyte T-tubule remodeling and dysregulated Ca²⁺ homeostasis, features associated with inherited and acquired cardiomyopathies and arrhythmogenic phenotypes in experimental models.
Junctophilin-2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the JPH2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the JPH2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the JPH2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Junctophilin-2 protein expression.
This CRISPR knockout system enables efficient generation of JPH2-deficient cell models for investigation of Junctophilin-2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting JPH2 exon(s) critical for Junctophilin-2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple JPH2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Junctophilin-2 CRISPR/Cas9 KO Plasmid (h) and Junctophilin-2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the JPH2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Junctophilin-2 HDR Plasmid (h) and Junctophilin-2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by JPH2 homology arms to support homology-directed repair at defined JPH2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.