Junctional SR Marker Antibody (VF1c): sc-58772

Junctional SR Marker Antibody (VF1c) is a mouse monoclonal IgG1 antibody that detects the Junctional SR Marker protein of rabbit origin by western blotting (WB) and immunofluorescence (IF). Anti-Junctional SR Marker antibody (VF1c) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Junctional SR Marker protein plays a crucial role in muscle physiology, particularly in the regulation of calcium release from the sarcoplasmic reticulum (SR), which is essential for muscle contraction. SR, a specialized type of smooth endoplasmic reticulum, surrounds myofibrils and forms dyad and triad junctions in cardiac and skeletal muscle, respectively. These junctions are critical for excitation-contraction coupling, as they facilitate the interaction between dihydropyridine receptors, which act as voltage sensors, and ryanodine receptors, which serve as calcium release channels. The precise regulation of calcium levels is vital for muscle function, and any disruption in this process can lead to muscle disorders. Junctional SR Marker (VF1c) monoclonal antibody serves as an invaluable tool for researchers studying the dynamics of calcium signaling and muscle contraction, providing insights into the underlying mechanisms of muscle physiology and potential pathologies.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Junctional SR Marker Antibody (VF1c) References:

1. The 90-kDa junctional sarcoplasmic reticulum protein forms an integral part of a supramolecular triad complex in skeletal muscle.  |  Froemming, GR., et al. 1999. Biochem Biophys Res Commun. 261: 603-9. PMID: 10441473
2. Junctional sarcoplasmic reticulum transmembrane proteins in the heart.  |  Müller, FU., et al. 2002. Basic Res Cardiol. 97 Suppl 1: I52-5. PMID: 12479235
3. Characterization of junctional sarcoplasmic reticulum Ca2+ content and release by short-term mechanical restitution in cardiac muscle.  |  Tameyasu, T., et al. 2004. Jpn J Physiol. 54: 209-19. PMID: 15541199
4. Effect of changes in action potential spike configuration, junctional sarcoplasmic reticulum micro-architecture and altered t-tubule structure in human heart failure.  |  Cannell, MB., et al. 2006. J Muscle Res Cell Motil. 27: 297-306. PMID: 16897575
5. Calcium is released from the junctional sarcoplasmic reticulum during cardiac muscle contraction.  |  Moravec, CS. and Bond, M. 1991. Am J Physiol. 260: H989-97. PMID: 2000992
6. Specific protein-protein interactions of calsequestrin with junctional sarcoplasmic reticulum of skeletal muscle.  |  Damiani, E. and Margreth, A. 1990. Biochem Biophys Res Commun. 172: 1253-9. PMID: 2123102
7. Characterization and ultrastructural localization of a novel 90-kDa protein unique to skeletal muscle junctional sarcoplasmic reticulum.  |  Guo, W., et al. 1994. J Biol Chem. 269: 28359-65. PMID: 7961775
8. Complex formation between junctin, triadin, calsequestrin, and the ryanodine receptor. Proteins of the cardiac junctional sarcoplasmic reticulum membrane.  |  Zhang, L., et al. 1997. J Biol Chem. 272: 23389-97. PMID: 9287354
9. Inositol polyphosphates regulate Ca2+ efflux in a cardiac membrane subtype distinct from junctional sarcoplasmic reticulum.  |  Quist, EE., et al. 2000. Arch Biochem Biophys. 384: 181-9. PMID: 11147829

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Junctional SR Marker Antibody (VF1c) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Junctional SR Marker Antibody (VF1c) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Junctional SR Marker Antibody (VF1c) Specifications

Alternate Product Name: Junctional SR Marker

Product Clone Name: VF1c

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids:

Epitope Target: Junctional SR Marker

Epitope Species: rabbit

Product Reactivity/Detection: Junctional SR Marker in skeletal muscle

Species Reactivity/Detection: rabbit

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IF

Vialing Information: Each vial contains 200 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Junctional SR Marker Antibody (VF1c): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin