JRAB Antibody (F-5): sc-376675

JRAB Antibody (F-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects JRAB protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). JRAB (F-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. JRAB protein, also known as junctional Rab13-binding protein or MICAL-like protein 2, consists of 904 amino acids and features one calponin-homology domain and one LIM zinc-binding domain, which are crucial for cellular transport function. JRAB plays a significant role in the interaction with Rab13 and Rab8, facilitating the transport of essential proteins such as claudin-1, occludin, and E-cadherin. This transport is vital for the assembly and maintenance of tight junctions and adherens junctions, which are critical for epithelial integrity and function. Dynamic turnover of these junctions through endocytic recycling is essential for epithelial morphogenesis during development and differentiation. JRAB and Rab13 interaction has been implicated in carcinoma metastasis, particularly in epithelial cell scattering, where both colocalize with F-Actin in lamellipodial structures, highlighting JRAB′s importance in both normal cellular processes and pathological conditions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

JRAB Antibody (F-5) References:

1. JRAB/MICAL-L2 is a junctional Rab13-binding protein mediating the endocytic recycling of occludin.  |  Terai, T., et al. 2006. Mol Biol Cell. 17: 2465-75. PMID: 16525024
2. Involvement of Rab13 and JRAB/MICAL-L2 in epithelial cell scattering.  |  Kanda, I., et al. 2008. Oncogene. 27: 1687-95. PMID: 17891173
3. The interaction of JRAB/MICAL-L2 with Rab8 and Rab13 coordinates the assembly of tight junctions and adherens junctions.  |  Yamamura, R., et al. 2008. Mol Biol Cell. 19: 971-83. PMID: 18094055
4. Regulation of epithelial cell adhesion and repulsion: role of endocytic recycling.  |  Nishimura, N. and Sasaki, T. 2008. J Med Invest. 55: 9-16. PMID: 18319540
5. Involvement of actinin-4 in the recruitment of JRAB/MICAL-L2 to cell-cell junctions and the formation of functional tight junctions.  |  Nakatsuji, H., et al. 2008. Mol Cell Biol. 28: 3324-35. PMID: 18332111
6. Identification and characterization of JRAB/MICAL-L2, a junctional Rab13-binding protein.  |  Nishimura, N. and Sasaki, T. 2008. Methods Enzymol. 438: 141-53. PMID: 18413246
7. Rab family small G proteins in regulation of epithelial apical junctions.  |  Nishimura, N. and Sasaki, T. 2009. Front Biosci (Landmark Ed). 14: 2115-29. PMID: 19273188
8. Rab13 regulates neurite outgrowth in PC12 cells through its effector protein, JRAB/MICAL-L2.  |  Sakane, A., et al. 2010. Mol Cell Biol. 30: 1077-87. PMID: 20008558

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-JRAB Antibody (F-5) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-JRAB Antibody (F-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

JRAB Antibody (F-5) Specifications

Alternate Product Name: JRAB

Product Clone Name: F-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 561-664

Epitope Target: JRAB

Epitope Species: human

Product Reactivity/Detection: JRAB

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for JRAB Antibody (F-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin