JMJD8 Double Nickase Plasmid (h): sc-406662-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- JMJD8 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- JMJD8 Double Nickase Plasmid (h) and JMJD8 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting JMJD8. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: JMJD8 Antibody (H-4): sc-515520
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
JMJD8 Double Nickase Plasmid (h) | sc-406662-NIC | 20 µg | $410.00 | |||
JMJD8 Double Nickase Plasmid (h2) | sc-406662-NIC-2 | 20 µg | $410.00 |
JMJD8 encodes a JmjC domain–containing protein implicated in regulation of protein homeostasis and stress-adaptive signaling. Human JMJD8 has been linked to endoplasmic reticulum–associated processes and modulation of receptor-driven pathways, including effects on folding/trafficking and quality-control outputs that shape cellular proteostasis. Through these functions, JMJD8 can influence proliferation, inflammatory signaling, and responses to metabolic or oxidative stress. Altered JMJD8 expression or pathway activity has been reported in multiple disease-relevant contexts, supporting investigation of its role in oncogenic signaling networks and cell-state regulation.
JMJD8 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the JMJD8 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within JMJD8. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt JMJD8 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of JMJD8-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.