Date published: 2025-12-8

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Janus Green B (CAS 2869-83-2)

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Alternate Names:
Diazin Green S
Application:
Janus Green B is a stain used for histology studies of mitochondria
CAS Number:
2869-83-2
Molecular Weight:
511.06
Molecular Formula:
C30H31N6•Cl
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Janus Green B is a stain that interacts with DNA and has been used for histology studies for mitochondrial staining, in which its oxidation and reduction reveals alterations to the electron transfer chain. The stain has been utilized in studies for effective and rapid staining of insect chordotonal organs and peripheral nerves, where the nerves appear dark blue in comparison to non stained tissue. With the use of Janus Green B, rabbit lymphatic vessel studies demonstrate that threadlike structures within the lymphatic vessels, known as Bonghan ducts, contain a high density of mitochondria in their cells.


Janus Green B (CAS 2869-83-2) References

  1. Interactions of Janus Green B with double stranded DNA and the determination of DNA based on the measurement of enhanced resonance light scattering.  |  Huang, CZ., et al. 2000. Analyst. 125: 1267-72. PMID: 10984922
  2. Studies on the mechanism of Janus green B staining of mitochondria. IV. Reduction of Janus green B by isolated cell fractions.  |  COOPERSTEIN, SJ., et al. 1960. Anat Rec. 138: 49-66. PMID: 13695346
  3. Novel threadlike structures (Bonghan ducts) inside lymphatic vessels of rabbits visualized with a Janus Green B staining method.  |  Lee, BC., et al. 2005. Anat Rec B New Anat. 286: 1-7. PMID: 16177995
  4. René Couteaux (1909-1999) and the morphological identification of synapses.  |  Tsuji, S. 2006. Biol Cell. 98: 503-9. PMID: 16842240
  5. Using Janus green B to study paraquat toxicity in rat liver mitochondria: role of ACE inhibitors (thiol and nonthiol ACEi).  |  Ghazi-Khansari, M., et al. 2006. Ann N Y Acad Sci. 1090: 98-107. PMID: 17384251
  6. Flow cytometric identification of two different rhodamine-123-stained mitochondrial populations in maize leaves.  |  Yang, J., et al. 2007. Protoplasma. 231: 249-52. PMID: 17922268
  7. Comparative analyses of cell disruption methods for mitochondrial isolation in high-throughput proteomics study.  |  Chaiyarit, S. and Thongboonkerd, V. 2009. Anal Biochem. 394: 249-58. PMID: 19622339
  8. Photo-Physical Characteristics of Janus Green B in Different Solvents and its Interaction Mechanism with Silver Nanoparticles.  |  Gawad, SAA., et al. 2024. J Fluoresc.. PMID: 38773030
  9. Janus Green B as a rapid, vital stain for peripheral nerves and chordotonal organs in insects.  |  Yack, JE. 1993. J Neurosci Methods. 49: 17-22. PMID: 7505866
  10. Binding of the cationic dye, Janus green B, as a measure of the specific surface area of crystalline silica in aqueous suspension.  |  Daniel, LN., et al. 1993. Toxicol Appl Pharmacol. 123: 62-7. PMID: 8236262

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Janus Green B, 5 g

sc-203740
5 g
$63.00

Janus Green B, 25 g

sc-203740A
25 g
$203.00

Janus Green B, 100 g

sc-203740B
100 g
$679.00

Janus Green B, 250 g

sc-203740C
250 g
$1332.00

Hi ,Is it possible to use (genus green B) for paraffin-prepared tissue sections for demonstration of mitochondria? Note that I have previously used one of your products which is LGR5 for demonstration of intestinal stem cell and gave good results

Asked by: Alhaaik AG
Thank you for your question. Janus Green B is a stain that interacts with DNA and is recommended for histology studies, including mitochondrial staining. We do not have any information specifically for paraffin embedded tissue sections.
Answered by: Tech Service
Date published: 2020-09-08

Will Janus Green B remain stable and effective if a 1% stock solution is frozen and then thawed before use? At the present moment, our stock is made fresh prior to being used.

Asked by: DHernandez
Unfortunately, we don't have any stability or freeze thaw cycle data of this product in solution form.
Answered by: Technical Support
Date published: 2020-09-08

What is the function of Janus green stain in mitochondrial staining?

Asked by: Swoosh
Thank you for your question. Janus Green B is a stain that interacts with DNA and has been used for histology studies including mitochondrial staining, in which its oxidation and reduction reveals alterations to the electron transfer chain. The stain has been utilized in studies for effective and rapid staining of insect chordotonal organs and peripheral nerves, where the nerves appear dark blue in comparison to non stained tissue.
Answered by: Technical Support
Date published: 2020-09-08

What is the solubility of this product?

Asked by: hawkeye11
Thank you for your question. Janus Green B, sc-203740, is soluble in both distilled water and alcohol (Ethanol).
Answered by: TechService7
Date published: 2020-09-08
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Rated 5 out of 5 by from Chaiyarit et alChaiyarit et al. (PubMed ID 19622339) used Janus Green B to examine protein purity resulting from the physical, mechanical and chemical disruption of MDCK cells. -SCBT Publication Review
Date published: 2015-03-12
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Janus Green B | CAS 2869-83-2 is rated 5.0 out of 5 by 1.
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