ITI-H2 Double Nickase Plasmid (h): sc-402683-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ITI-H2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ITI-H2 Double Nickase Plasmid (h) and ITI-H2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ITIH2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ITI-H2 Double Nickase Plasmid (h) | sc-402683-NIC | 20 µg | $410.00 | |||
ITI-H2 Double Nickase Plasmid (h2) | sc-402683-NIC-2 | 20 µg | $410.00 |
ITIH2 encodes inter-α-trypsin inhibitor heavy chain 2 (ITI-H2), a secreted extracellular matrix–associated glycoprotein that contributes to stabilization of hyaluronan-rich matrices through interactions with inter-α-inhibitor components and related transfer reactions that support tissue organization. By modulating the physical properties of the pericellular matrix, ITI-H2 influences cell adhesion, migration, and inflammatory remodeling processes relevant to wound response and stromal biology. Altered expression or processing of inter-α-inhibitor family proteins, including ITIH2, has been reported across inflammatory conditions and multiple cancer contexts, where extracellular matrix composition can shape invasion and immune cell trafficking. ITIH2 therefore serves as a useful node for studying ECM-driven signaling crosstalk, protease regulation, and microenvironment-dependent phenotypes in human cell models.
ITI-H2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ITIH2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ITIH2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ITIH2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ITIH2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.