IST1 Double Nickase Plasmid (h): sc-411346-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IST1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IST1 Double Nickase Plasmid (h) and IST1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IST1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IST1 Double Nickase Plasmid (h) | sc-411346-NIC | 20 µg | $410.00 |
Human IST1 (increased sodium tolerance 1 homolog) encodes a component of the ESCRT-III–associated machinery that coordinates endosomal membrane remodeling and fission. IST1 interacts with CHMP proteins and the AAA ATPase VPS4 to support multivesicular body biogenesis, endosomal sorting, and abscission during cytokinetic completion. Through its role in ESCRT-dependent membrane scission, IST1 influences receptor trafficking and proteostasis, processes frequently perturbed in cancer and neurodegeneration. Altered ESCRT function has also been linked to defects in genome stability and inflammatory signaling, making IST1 a useful entry point for studying membrane trafficking–coupled stress responses.
IST1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IST1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IST1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IST1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IST1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.