ISCA1 Double Nickase Plasmid (m): sc-427272-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ISCA1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ISCA1 Double Nickase Plasmid (m) and ISCA1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Isca1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ISCA1 Double Nickase Plasmid (m) | sc-427272-NIC | 20 µg | $410.00 | |||
ISCA1 Double Nickase Plasmid (m2) | sc-427272-NIC-2 | 20 µg | $410.00 |
Mouse ISCA1 (Isca1) encodes a mitochondrial A-type carrier implicated in iron–sulfur (Fe–S) cluster maturation, supporting the biogenesis of [4Fe–4S] cofactors required by respiratory chain components and numerous metabolic enzymes. Through its role in late-stage Fe–S assembly, ISCA1 contributes to oxidative phosphorylation, redox homeostasis, and maintenance of mitochondrial function under variable cellular iron conditions. Disruption of Fe–S cluster pathways can destabilize mitochondrial genome maintenance and electron transport, linking ISCA1-related dysfunction to bioenergetic stress phenotypes relevant to neurodevelopmental and neuromuscular disease mechanisms. As a result, Isca1 is frequently studied in the context of mitochondrial metabolism, stress signaling, and Fe–S-dependent enzyme networks.
ISCA1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Isca1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Isca1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Isca1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Isca1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.