IRP-2 Double Nickase Plasmid (m): sc-425717-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IRP-2 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IRP-2 Double Nickase Plasmid (m) and IRP-2 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Ireb2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IRP-2 Antibody (4G11): sc-33680
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IRP-2 Double Nickase Plasmid (m) | sc-425717-NIC | 20 µg | $410.00 |
Mouse Ireb2 encodes iron regulatory protein 2 (IRP-2), an RNA-binding factor that post-transcriptionally controls iron homeostasis by recognizing iron-responsive elements in target mRNAs such as transferrin receptor 1 and ferritin. Through iron- and oxygen-dependent regulation of IRP-2 stability, Ireb2 integrates cellular iron availability with pathways governing mitochondrial metabolism, redox balance, and heme/iron–sulfur cluster biogenesis. Perturbation of IRP-2 activity can shift labile iron pools and oxidative stress responses, linking iron dyshomeostasis to neurodegeneration, anemia-related phenotypes, and inflammatory signaling in multiple tissues. These properties make Ireb2 a useful locus for mechanistic studies of iron-dependent gene regulation, ferroptosis susceptibility, and metabolic adaptation in mouse models and cultured cells.
IRP-2 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Ireb2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Ireb2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Ireb2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Ireb2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.