IRE1β CRISPR/Cas9 KO Plasmid (m): sc-424138
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IRE1β CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the IRE1β genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IRE1β CRISPR/Cas9 KO Plasmid (m) | sc-424138 | 20 µg | $397.00 |
Overview
Ern2 encodes inositol-requiring enzyme 1 beta (IRE1β), an endoplasmic reticulum (ER)–resident transmembrane kinase/endoribonuclease that functions as a stress sensor within the unfolded protein response (UPR). Upon ER stress, IRE1β contributes to ER homeostasis through regulated IRE1-dependent RNA decay and modulation of Xbp1 mRNA splicing, influencing secretory capacity, epithelial differentiation, and inflammatory signaling. In mouse, Ern2 is enriched in mucosal and secretory epithelia, where it helps coordinate protein folding demand with barrier and mucin-related programs. Dysregulation of ER stress pathways involving IRE1β is relevant to studies of intestinal inflammation, mucus layer defects, host–microbe interactions, and metabolic stress phenotypes in epithelial tissues.
IRE1β CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ern2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ern2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ern2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish IRE1β protein expression.
This CRISPR knockout system enables efficient generation of Ern2-deficient cell models for investigation of IRE1β signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ern2 exon(s) critical for IRE1β function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ern2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by IRE1β CRISPR/Cas9 KO Plasmid (m) and IRE1β CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ern2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by IRE1β HDR Plasmid (m) and IRE1β HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ern2 homology arms to support homology-directed repair at defined Ern2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.