IQGAP1 Double Nickase Plasmid (h): sc-400597-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IQGAP1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IQGAP1 Double Nickase Plasmid (h) and IQGAP1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IQGAP1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IQGAP1 Antibody (C-9): sc-376021
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IQGAP1 Double Nickase Plasmid (h) | sc-400597-NIC | 20 µg | $410.00 | |||
IQGAP1 Double Nickase Plasmid (h2) | sc-400597-NIC-2 | 20 µg | $410.00 |
IQGAP1 encodes IQGAP1, a multidomain scaffolding protein that integrates signals from Rho-family GTPases, calmodulin, and cytoskeletal regulators to coordinate actin dynamics, cell polarity, and membrane trafficking. IQGAP1 links adhesion complexes and the actin cytoskeleton to signaling pathways including MAPK/ERK and Wnt/β-catenin, thereby influencing migration, proliferation, and cytokinesis. Through interactions with proteins such as CDC42, RAC1, β-catenin, E-cadherin, and components of the exocyst, IQGAP1 helps organize spatially restricted signaling at the leading edge and cell–cell junctions. Dysregulation of IQGAP1 expression or localization has been associated with altered epithelial integrity, invasive behavior, and oncogenic signaling in multiple cancer-relevant contexts, making it a useful node for mechanistic studies of metastasis-related processes.
IQGAP1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IQGAP1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IQGAP1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IQGAP1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IQGAP1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.