Integrin αIIb/ITGA2B/CD41 CRISPR/Cas9 KO Plasmid (m): sc-421160

Overview

Itga2b encodes integrin αIIb (CD41), which pairs with integrin β3 (CD61) to form the platelet fibrinogen receptor αIIbβ3, a central mediator of platelet adhesion and aggregation. Upon activation, αIIbβ3 undergoes inside-out and outside-in signaling that links extracellular matrix ligands such as fibrinogen and von Willebrand factor to cytoskeletal remodeling, focal adhesion dynamics, and Src/FAK-dependent signaling pathways. In mice, Itga2b expression marks megakaryocyte lineage commitment and supports platelet production and hemostatic responses. Dysregulated integrin signaling and altered platelet activation are broadly relevant to studies of thrombocytopenia, bleeding phenotypes, inflammation-associated thrombosis, and platelet–immune crosstalk in vascular disease models.

Integrin αIIb/ITGA2B/CD41 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Itga2b gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Itga2b together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Itga2b open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Integrin αIIb/ITGA2B/CD41 protein expression.

This CRISPR knockout system enables efficient generation of Itga2b-deficient cell models for investigation of Integrin αIIb/ITGA2B/CD41 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting Itga2b exon(s) critical for Integrin αIIb/ITGA2B/CD41 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple Itga2b genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by Integrin αIIb/ITGA2B/CD41 CRISPR/Cas9 KO Plasmid (m) and Integrin αIIb/ITGA2B/CD41 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Itga2b locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by Integrin αIIb/ITGA2B/CD41 HDR Plasmid (m) and Integrin αIIb/ITGA2B/CD41 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Itga2b homology arms to support homology-directed repair at defined Itga2b target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.