Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Integrin β5/ITGB5 Double Nickase Plasmid (h): sc-401017-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Integrin β5/ITGB5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Integrin β5/ITGB5 Double Nickase Plasmid (h) and Integrin β5/ITGB5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ITGB5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Integrin β5/ITGB5 Antibody (F-5): sc-398214
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Integrin β5/ITGB5 Double Nickase Plasmid (h) | sc-401017-NIC | 20 µg | $410.00 | |||
Integrin β5/ITGB5 Double Nickase Plasmid (h2) | sc-401017-NIC-2 | 20 µg | $410.00 |
ITGB5 encodes integrin β5, a transmembrane adhesion receptor that primarily heterodimerizes with integrin αV to form αVβ5, linking extracellular matrix ligands to the actin cytoskeleton. This receptor participates in focal adhesion assembly and bidirectional signaling that regulates cell adhesion, spreading, migration, and mechanotransduction through pathways such as FAK/SRC, PI3K–AKT, and MAPK. αVβ5 also contributes to endocytosis-associated signaling and can influence TGF-β activation dynamics depending on cellular context. Dysregulated ITGB5 expression or integrin signaling has been associated with altered invasive behavior, angiogenic responses, and remodeling programs observed in multiple cancer and fibrotic disease models.
Integrin β5/ITGB5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ITGB5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ITGB5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ITGB5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ITGB5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.