Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Integrin β3 Antibody (B-7): sc-46655. Western blot analysis of Integrin β3 expression in Jurkat (A), TK-1 (B), C2C12 (C), Neuro-2A (D) and HEL 92.1.7 (E) whole cell lysates.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Integrin β3/ITGB3/CD61 Double Nickase Plasmid (m): sc-421175-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Integrin β3/ITGB3/CD61 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Integrin β3/ITGB3/CD61 Double Nickase Plasmid (m) and Integrin β3/ITGB3/CD61 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Itgb3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Integrin β3/ITGB3/CD61 Antibody (D-11): sc-365679
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Integrin β3/ITGB3/CD61 Double Nickase Plasmid (m) | sc-421175-NIC | 20 µg | $410.00 | |||
Integrin β3/ITGB3/CD61 Double Nickase Plasmid (m2) | sc-421175-NIC-2 | 20 µg | $410.00 |