Int-6 Double Nickase Plasmid (h2): sc-405759-NIC-2
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Int-6 Double Nickase Plasmid (h2) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Int-6 Double Nickase Plasmid (h2) and Int-6 Double Nickase Plasmid (h22) encode distinct paired gRNA designs targeting EIF3E. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Int-6 Antibody (A-11): sc-133251
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Int-6 Double Nickase Plasmid (h2) | sc-405759-NIC-2 | 20 µg | $410.00 |
Human EIF3E (Int-6) encodes a core subunit of the eukaryotic translation initiation factor 3 (eIF3) complex that helps coordinate 40S ribosomal subunit recruitment and regulates translation initiation, with downstream effects on proteostasis and cell-cycle progression. Int-6 has been linked to crosstalk between translational control and protein turnover pathways, including interactions with the ubiquitin–proteasome system, and is studied in contexts such as stress-responsive remodeling of mRNA translation. Altered EIF3E expression or function is associated with dysregulated growth signaling and has been reported in multiple cancer-related datasets, supporting its utility for investigating oncogenic translation programs and genome stability. Gene editing of EIF3E enables mechanistic studies of eIF3 complex integrity, selective mRNA translation, and pathway dependencies in human cell models using knockout, knock-in, or domain-specific perturbations.
Int-6 Double Nickase Plasmid (h2) consists of a matched pair of plasmids engineered for high-specificity editing of the EIF3E locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EIF3E. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EIF3E function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EIF3E-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.