Int-6 Antibody (A-8): sc-74505

Int-6 Antibody (A-8) is a mouse monoclonal IgG2a antibody that detects Int-6 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Int-6, also known as eIF3e or eIF3-p46, plays a crucial role in regulating translation and protein degradation by interacting with several key complexes, including the eukaryotic translation initiation factor 3 (eIF3), the proteasome regulatory lid, and the constitutive photomorphogenesis 9 signalosome. The eIF3 complex is essential for the assembly of 40S ribosomal subunits on mRNA, which is vital for initiating protein synthesis. Notably, Int-6 is a significant site of integration for the mouse mammary tumor virus (MMTV) in murine tumors, highlighting Int-6′s potential role in cancer biology. Studies have shown that reducing Int-6 expression through RNA interference in HeLa cells can lead to defects in mitosis, including issues with spindle formation, chromosome segregation, and cytokinesis, which are associated with the inhibition of cyclin B-Cdk1 kinase activity. This inhibition is attributed to a prolonged phosphorylated state of Cdk1, underscoring Int-6′s importance in cell cycle regulation and potential implications in tumorigenesis.

The preferred Int-6 antibody is Int-6 Antibody (A-11): sc-133251.