INSIG-2 Double Nickase Plasmid (h): sc-402255-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- INSIG-2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- INSIG-2 Double Nickase Plasmid (h) and INSIG-2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting INSIG2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
INSIG-2 Double Nickase Plasmid (h) | sc-402255-NIC | 20 µg | $410.00 | |||
INSIG-2 Double Nickase Plasmid (h2) | sc-402255-NIC-2 | 20 µg | $410.00 |
INSIG2 encodes INSIG-2, an endoplasmic reticulum membrane protein that restrains cholesterol and fatty acid biosynthesis by regulating SREBP processing. INSIG-2 binds sterol-sensing SCAP and promotes ER retention of the SCAP–SREBP complex under sterol-replete conditions, thereby limiting transcriptional programs controlling lipid metabolism and membrane biogenesis. Through interactions with ubiquitin ligase machinery, INSIG-2 also contributes to the regulated turnover of HMG-CoA reductase, linking sterol availability to mevalonate pathway flux. Altered INSIG2 expression or regulation has been associated with dyslipidemia-related traits, insulin resistance, and metabolic disease phenotypes, making it a useful node for studying lipid homeostasis in human cells.
INSIG-2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the INSIG2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within INSIG2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt INSIG2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of INSIG2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.