INCENP Antibody (B-4): sc-376514

INCENP Antibody (B-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects INCENP of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-INCENP antibody (B-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. INCENP, also known as inner centromere protein, plays a pivotal role in chromosome segregation during mitosis, ensuring that replicated chromosomes are accurately distributed to daughter cells. INCENP is essential for the proper functioning of the kinetochore, a structure that forms at the centromere and is critical for the attachment of microtubules during cell division. INCENP is involved in the formation of the chromosomal passenger complex, which also includes Aurora B kinase, and is crucial for regulating various mitotic processes, including the onset of cytokinesis. INCENP′s ability to form homodimers or heterodimers allows interactions with other key proteins such as AURKB, AURKC, CBX3, and β Tubulin, facilitating localization to the mitotic spindle and metaphase chromosomes, and during anaphase, to the equatorial cortex. INCENP′s dynamic localization and interactions underscore its importance in maintaining genomic stability and proper cell division, making INCENP a valuable target for research in cancer biology and cell cycle regulation.

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INCENP Antibody (B-4) References:

1. INCENP binds directly to tubulin and requires dynamic microtubules to target to the cleavage furrow.  |  Wheatley, SP., et al. 2001. Exp Cell Res. 262: 122-7. PMID: 11139336
2. The chromosomal passenger complex takes center stage during mitosis.  |  Vernos, I. 2004. Dev Cell. 7: 145-6. PMID: 15296708
3. Direct association with inner centromere protein (INCENP) activates the novel chromosomal passenger protein, Aurora-C.  |  Li, X., et al. 2004. J Biol Chem. 279: 47201-11. PMID: 15316025
4. Recruitment of MKLP1 to the spindle midzone/midbody by INCENP is essential for midbody formation and completion of cytokinesis in human cells.  |  Zhu, C., et al. 2005. Biochem J. 389: 373-81. PMID: 15796717
5. Mechanism of Aurora B activation by INCENP and inhibition by hesperadin.  |  Sessa, F., et al. 2005. Mol Cell. 18: 379-91. PMID: 15866179
6. Tetraploidy/aneuploidy and stem cells in cancer promotion: The role of chromosome passenger proteins.  |  Nguyen, HG. and Ravid, K. 2006. J Cell Physiol. 208: 12-22. PMID: 16331679
7. Phosphoproteome analysis of the human mitotic spindle.  |  Nousiainen, M., et al. 2006. Proc Natl Acad Sci U S A. 103: 5391-6. PMID: 16565220
8. Structural basis for binding of aurora-AG198N- INCENP complex: MD simulations and free energy calculations.  |  Tanneeru, K. and Guruprasad, L. 2013. Protein Pept Lett. 20: 1246-56. PMID: 23848594
9. A small molecule identified through an in silico screen inhibits Aurora B-INCENP interaction.  |  Unsal, E., et al. 2016. Chem Biol Drug Des. 88: 783-794. PMID: 27390292
10. The role of the CDCA gene family in ovarian cancer.  |  Chen, C., et al. 2020. Ann Transl Med. 8: 190. PMID: 32309337
11. Bipartite binding interface recruiting HP1 to chromosomal passenger complex at inner centromeres.  |  Sako, K., et al. 2024. J Cell Biol. 223: PMID: 38781028
12. INCENP centromere and spindle targeting: identification of essential conserved motifs and involvement of heterochromatin protein HP1.  |  Ainsztein, AM., et al. 1998. J Cell Biol. 143: 1763-74. PMID: 9864353

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-INCENP Antibody (B-4) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-INCENP Antibody (B-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

INCENP Antibody (B-4) Specifications

Alternate Product Name: INCENP

Product Clone Name: B-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 766-918

Epitope Target: INCENP

Epitope Species: human

Product Reactivity/Detection: INCENP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for INCENP Antibody (B-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin