ImmunoCruz^® rabbit LSAB Staining System: sc-2051

The ImmunoCruz rabbit LSAB Staining System is an advanced diagnostic kit designed for the immunohistochemical (IHC) analysis of antigens in tissue sections, utilizing the labeled streptavidin-biotin (LSAB) method, which is known for its enhanced sensitivity and specificity. This system is tailored for use with primary antibodies raised in rabbits, making it particularly suited for detecting a wide range of antigens with high fidelity. The LSAB staining method operates on a three-tiered approach: first, a primary rabbit antibody is applied to the tissue, where it binds specifically to its target antigen. Next, a biotinylated secondary antibody is introduced, which recognizes and binds to the rabbit primary antibody. The pivotal component of the system is the streptavidin-biotinylated enzyme complex, typically horseradish peroxidase (HRP) or alkaline phosphatase. Streptavidin, a protein with high affinity for biotin, forms a bridge between the biotinylated secondary antibody and the enzyme, effectively amplifying the signal. Upon addition of a chromogenic substrate, the enzyme catalyzes a colorimetric reaction that visibly marks the antigen's location within the tissue. This system is extensively used in research environments to study tissue morphology and protein localization, aiding in understanding biological processes and disease mechanisms without any clinical or therapeutic applications.