ImmunoCruz goat ABC Staining SystemSystem includes 1.0 ml normal mouse blocking serum, 250 µg mouse monoclonal anti-goat IgG-Biotin secondary, avidin and biotinylated horseradish peroxidase (ABC reagents), peroxidase substrate, DAB chromogen and DAB dilution buffer.
System includes 1.0 ml normal mouse blocking serum, 250 µg mouse monoclonal anti-goat IgG-Biotin secondary, avidin and biotinylated horseradish peroxidase (ABC reagents), peroxidase substrate, DAB chromogen and DAB dilution buffer.
Tissue sections are first incubated with primary antibody. Biotinylated secondary antibody is added, followed by the addition of preformed ABC reagent. The target protein is visualized by incubation in peroxidase substrate.
Number of slides: 200
While we continue to offer the ImmunoCruz® ABC Kit, we highly recommend using Direct HRP Conjugated Primary Monoclonal Antibodies for optimal IHC Staining. See the product page for antibody of interest for HRP Direct Conjugate antibody availability.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
How long can the prepared DAB solution remain at 5C? Is the solution light sensitive?
Asked by: MrImmune
Thank you for your question. We recommend to prepare the peroxidase substrate (DAB) solution fresh each time before use.
Answered by: Technical Support
Date published: 2019-09-10
What is the volume of DAB in this kit? What is the ratio of DAB to H2O2 to make up the working solution?
Asked by: Histogal
Thank you for your question. This information is provided on the product data sheet that can be downloaded using the link above on this product page. Also, see below.
1.0 ml 50x DAB chromogen is provided
Preparation of Working Solutions:
Peroxidase substrate: In substrate mixing bottle (yellow cap), combine 1.7 ml distilled H2O, 3 drops 15x substrate buffer, 1 drop 50x DAB chromogen and 1 drop 50x peroxidase substrate. Sufficient for 15–20 slides.
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn
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