IMMP2L Double Nickase Plasmid (h): sc-413529-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IMMP2L Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IMMP2L Double Nickase Plasmid (h) and IMMP2L Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IMMP2L. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IMMP2L Double Nickase Plasmid (h) | sc-413529-NIC | 20 µg | $410.00 |
IMMP2L encodes an inner mitochondrial membrane peptidase subunit that contributes to the maturation of select nuclear-encoded mitochondrial proteins following their import, supporting mitochondrial proteostasis and efficient oxidative phosphorylation. By influencing respiratory chain function and mitochondrial quality control, IMMP2L impacts cellular redox balance, ATP production, and stress-responsive signaling pathways linked to mitochondrial homeostasis. Genetic variation and structural alterations at the IMMP2L locus have been associated with neurodevelopmental and neuropsychiatric phenotypes, making it relevant for mechanistic studies of mitochondria-dependent neuronal function. In biomedical research, IMMP2L perturbation is used to interrogate how mitochondrial processing defects reshape bioenergetics, ROS signaling, and downstream transcriptional programs.
IMMP2L Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IMMP2L locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IMMP2L. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IMMP2L function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IMMP2L-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.