IMMP1L Double Nickase Plasmid (h): sc-414955-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IMMP1L Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IMMP1L Double Nickase Plasmid (h) and IMMP1L Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IMMP1L. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IMMP1L Double Nickase Plasmid (h) | sc-414955-NIC | 20 µg | $410.00 |
IMMP1L encodes an inner mitochondrial membrane peptidase subunit that participates in the maturation of imported mitochondrial proteins by processing signal peptides after translocation into the inner membrane. By supporting mitochondrial protein quality control and oxidative phosphorylation competence, IMMP1L helps maintain bioenergetic homeostasis and limits secondary stress responses linked to mitochondrial dysfunction, including altered reactive oxygen species handling and mitophagy dynamics. Genetic and functional studies have associated IMMP1L variation with neurodevelopmental and neuropsychiatric phenotypes, consistent with the high sensitivity of neurons to perturbations in mitochondrial proteostasis. IMMP1L is therefore relevant for mechanistic research on mitochondrial processing pathways and how inner-membrane protease activity shapes cellular metabolism and stress adaptation.
IMMP1L Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IMMP1L locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IMMP1L. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IMMP1L function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IMMP1L-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.