IL-6 Antibody (AH65): sc-53865

IL-6 Antibody (AH65) is a mouse monoclonal IgG1 antibody that detects interleukin-6 (IL-6) in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). IL-6 is a multifunctional cytokine composed of 212 amino acids, playing a pivotal role in immune response, host defense, and hematopoiesis. IL-6 is constitutively expressed by epidermal Langerhans cells, with expression further induced in stimulated keratinocytes, highlighting importance in skin immunity and inflammation. The IL-6 receptor forms a trimeric complex that includes an IL-6-specific α chain and a homodimer of the gp130 glycoprotein, which is shared among several other cytokine receptors. Upon binding of IL-6, this complex triggers the homodimerization of gp130, leading to the activation of Janus kinases JAK1 and JAK2. These kinases subsequently phosphorylate Stat3, facilitating translocation to the nucleus where Stat3 drives the transcription of responsive genes, thereby influencing various biological processes. Additionally, IL-6 activates the Ras/MAP kinase pathway, which plays a crucial role in regulating NFIL6 transcription, further underscoring IL-6 significance in cellular signaling and immune regulation. Anti-IL-6 antibody (AH65) serves as an essential tool for researchers studying IL-6 roles in health and disease.

The preferred IL-6 antibody is IL-6 Antibody (E-4): sc-28343.