IL-33 Antibody (4E9): sc-130625

IL-33 Antibody (4E9) is a mouse monoclonal IgG2b antibody that detects IL-33 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-IL-33 antibody (4E9) is available as a non-conjugated format. IL-33 plays a crucial role in the immune response, acting as an alarmin that signals tissue damage and promotes inflammation. IL-33 is primarily expressed in epithelial cells and is released upon cellular stress or injury, which is vital for initiating protective immune responses. IL-33 in the extracellular space can activate various immune cells, including T helper 2 cells, mast cells, and eosinophils, thereby influencing the development of allergic and autoimmune diseases. Understanding IL-33 function is essential for developing therapeutic strategies targeting inflammatory conditions, as IL-33 dysregulation is implicated in diseases such as asthma, rheumatoid arthritis, and inflammatory bowel disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

IL-33 Antibody (4E9) References:

1. Molecular characterization of NF-HEV, a nuclear factor preferentially expressed in human high endothelial venules.  |  Baekkevold, ES., et al. 2003. Am J Pathol. 163: 69-79. PMID: 12819012
2. IL-33 and ST2 comprise a critical biomechanically induced and cardioprotective signaling system.  |  Sanada, S., et al. 2007. J Clin Invest. 117: 1538-49. PMID: 17492053
3. Soluble ST2 blocks interleukin-33 signaling in allergic airway inflammation.  |  Hayakawa, H., et al. 2007. J Biol Chem. 282: 26369-80. PMID: 17623648
4. Cutting edge: The ST2 ligand IL-33 potently activates and drives maturation of human mast cells.  |  Allakhverdi, Z., et al. 2007. J Immunol. 179: 2051-4. PMID: 17675461
5. IL-33 can promote survival, adhesion and cytokine production in human mast cells.  |  Iikura, M., et al. 2007. Lab Invest. 87: 971-8. PMID: 17700564
6. The IL-1 receptor accessory protein (AcP) is required for IL-33 signaling and soluble AcP enhances the ability of soluble ST2 to inhibit IL-33.  |  Palmer, G., et al. 2008. Cytokine. 42: 358-64. PMID: 18450470
7. A novel IL-1 family cytokine, IL-33, potently activates human eosinophils.  |  Cherry, WB., et al. 2008. J Allergy Clin Immunol. 121: 1484-90. PMID: 18539196
8. Nuclear interleukin-33 is generally expressed in resting endothelium but rapidly lost upon angiogenic or proinflammatory activation.  |  Küchler, AM., et al. 2008. Am J Pathol. 173: 1229-42. PMID: 18787100
9. The IL-33/ST2 pathway: therapeutic target and novel biomarker.  |  Kakkar, R. and Lee, RT. 2008. Nat Rev Drug Discov. 7: 827-40. PMID: 18827826
10. IL-33 signaling in sensory neurons promotes dry skin itch.  |  Trier, AM., et al. 2022. J Allergy Clin Immunol. 149: 1473-1480.e6. PMID: 34560104
11. Emerging Functions of IL-33 in Homeostasis and Immunity.  |  Dwyer, GK., et al. 2022. Annu Rev Immunol. 40: 15-43. PMID: 34985928
12. Oxidised IL-33 drives COPD epithelial pathogenesis via ST2-independent RAGE/EGFR signalling complex.  |  Strickson, S., et al. 2023. Eur Respir J. 62: PMID: 37442582

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-IL-33 Antibody (4E9) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-IL-33 Antibody (4E9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

IL-33 Antibody (4E9) Specifications

Alternate Product Name: Interleukin 33

Product Clone Name: 4E9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b

Amino Acids: 112-270

Epitope Target: IL-33

Epitope Species: human

Product Reactivity/Detection: IL-33

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 50 µg IgG_2b in 500 µl of PBS with < 0.1% sodium azide, 0.1% gelatin and 1% glycerol

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for IL-33 Antibody (4E9): N/A

The preferred IL-33 antibody is IL-33 Antibody (Nessy-1): sc-517600.