IL-2Rβ CRISPR Activation Plasmid (h): sc-403250-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IL-2Rβ CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- IL-2Rβ CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by IL-2Rβ CRISPR Activation Plasmid (h) and IL-2Rβ CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the IL2RB transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IL-2Rβ Antibody (C-2): sc-166427
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-2Rβ CRISPR Activation Plasmid (h) | sc-403250-ACT | 20 µg | $397.00 | |||
IL-2Rβ CRISPR Activation Plasmid (h2) | sc-403250-ACT-2 | 20 µg | $397.00 |
IL2RB encodes the human interleukin-2 receptor beta chain (IL‑2Rβ; CD122), a signaling subunit shared by the IL‑2 and IL‑15 receptor complexes that is essential for lymphocyte activation and homeostasis. Upon cytokine binding, IL‑2Rβ cooperates with IL2RG and IL2RA/IL15RA to initiate JAK1/JAK3-dependent phosphorylation cascades that activate STAT5 and intersect with PI3K–AKT and MAPK pathways. This signaling coordinates T cell proliferation, differentiation, and survival as well as NK cell development and effector function. Dysregulated IL2RB activity or expression can perturb immune tolerance and cytotoxic responses, making it a widely used node for mechanistic studies of immune regulation and inflammatory disease biology.
IL-2Rβ CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous IL2RB expression without altering the underlying DNA sequence.
IL-2Rβ CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the IL2RB locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the IL2RB transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous IL-2Rβ expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native IL2RB locus and enabling the study of IL-2Rβ-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of IL-2Rβ pathway restoration in tumor cells with silenced or reduced IL2RB expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.