Il-28a Double Nickase Plasmid (m2): sc-437298-NIC-2
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Il-28a CRISPR/Cas9 KOPlasmid Double Nickase Plasmid (m2) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Il-28a CRISPR/Cas9 KOPlasmid Double Nickase Plasmid (m2) and Il-28a CRISPR/Cas9 KOPlasmid Double Nickase Plasmid (m22) encode distinct paired gRNA designs targeting Ifnl2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Il-28a Double Nickase Plasmid (m2) | sc-437298-NIC-2 | 20 µg | $410.00 |
Mouse Gbp2b encodes a guanylate-binding protein family member implicated in interferon-driven cell-intrinsic immunity, where it contributes to antimicrobial restriction and coordination of innate immune signaling downstream of JAK–STAT and interferon-stimulated gene programs. The Il-28a protein (also known as IFN-λ2) is a type III interferon that signals through the IFNLR1/IL10RB receptor complex to activate STAT1/STAT2–IRF9 (ISGF3) transcriptional responses, shaping epithelial and mucosal antiviral defenses and modulating inflammatory cytokine networks. Dysregulation of these pathways is relevant to studies of viral susceptibility, barrier immunity, and chronic inflammatory phenotypes, including airway and gastrointestinal inflammation. Gene editing of Gbp2b or Il-28a in mouse models supports mechanistic dissection of interferon crosstalk, pathogen-host interactions, and downstream transcriptional programs using infection challenges, transcriptomics, and immune cell profiling.
Il-28a CRISPR/Cas9 KOPlasmid Double Nickase Plasmid (m2) consists of a matched pair of plasmids engineered for high-specificity editing of the Ifnl2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Ifnl2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Ifnl2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Ifnl2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.