Date published: 2026-5-16

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IL-14 Antibody (16C5): sc-80994

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Datasheets
  • IL-14 Antibody (16C5) is a mouse monoclonal IgG2a IL-14 antibody, cited in 1 publications, provided at 200 µg/ml
  • raised against recombinant IL-14 of human origin
  • IL-14 Antibody (16C5) is recommended for detection of IL-14 of mouse, rat and human origin by WB and IP
  • Anti-IL-14 Antibody (16C5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for IL-14 Antibody (16C5). This work is in progress.
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IL-14 Antibody (16C5) is a mouse monoclonal IgG2a antibody that detects IL-14 of mouse, rat, and human origin by western blotting (WB) and immunoprecipitation (IP). Anti-IL-14 antibody (16C5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. IL-14, also known as high-molecular-weight B-cell growth factor (HMW-BCGF), is crucial for the rapid proliferation of aggressive B-cell type non-Hodgkin′s lymphoma, highlighting its importance in cancer biology and potential therapeutic targeting. IL-14 is involved in the regulation of immune responses, particularly in B-cell activation and differentiation, which is vital for understanding immune system dynamics and developing treatments for related disorders. IL-14 interactions with other cytokines, such as IL-19 and IL-20, further underscore a role in modulating immune responses, as these cytokines are implicated in processes like melanoma differentiation and epidermal function in psoriasis. Anti-IL-14 antibody (16C5) enables researchers to gain insights into the complex signaling pathways that govern B-cell behavior and the broader implications for immune-related diseases.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

IL-14 Antibody (16C5) References:

  1. Interleukin 21 and its receptor are involved in NK cell expansion and regulation of lymphocyte function.  |  Parrish-Novak, J., et al. 2000. Nature. 408: 57-63. PMID: 11081504
  2. Interleukin 20: discovery, receptor identification, and role in epidermal function.  |  Blumberg, H., et al. 2001. Cell. 104: 9-19. PMID: 11163236
  3. Cloning, expression and initial characterization of interleukin-19 (IL-19), a novel homologue of human interleukin-10 (IL-10).  |  Gallagher, G., et al. 2000. Genes Immun. 1: 442-50. PMID: 11196675
  4. Cytokines: IL-21 joins the gamma(c)-dependent network?  |  Vosshenrich, CA. and Di Santo, JP. 2001. Curr Biol. 11: R175-7. PMID: 11267886
  5. Identification of B-cell growth factors (interleukin-14; high molecular weight-B-cell growth factors) in effusion fluids from patients with aggressive B-cell lymphomas.  |  Ford, R., et al. 1995. Blood. 86: 283-93. PMID: 7795235

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

IL-14 Antibody (16C5)

sc-80994
200 µg/ml
$322.00

IL-14 Antibody (16C5) AC

sc-80994 AC
500 µg/ml, 25% agarose
$424.00

IL-14 Antibody (16C5) HRP

sc-80994 HRP
200 µg/ml
$322.00

IL-14 Antibody (16C5) FITC

sc-80994 FITC
200 µg/ml
$336.00

IL-14 Antibody (16C5) PE

sc-80994 PE
200 µg/ml
$349.00

IL-14 Antibody (16C5) Alexa Fluor® 488

sc-80994 AF488
200 µg/ml
$364.00

IL-14 Antibody (16C5) Alexa Fluor® 546

sc-80994 AF546
200 µg/ml
$364.00

IL-14 Antibody (16C5) Alexa Fluor® 594

sc-80994 AF594
200 µg/ml
$364.00

IL-14 Antibody (16C5) Alexa Fluor® 647

sc-80994 AF647
200 µg/ml
$364.00

IL-14 Antibody (16C5) Alexa Fluor® 680

sc-80994 AF680
200 µg/ml
$364.00

IL-14 Antibody (16C5) Alexa Fluor® 790

sc-80994 AF790
200 µg/ml
$364.00

I am using IL-14 (16C5): sc-80994 antibody for immunoprecipitation, how much antibody should I use?

Asked by: Trav11
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Good for Western blot[Employee of scbt] Antibody works for Western blot with CCSP-RAGE TG mouse lung homogenates in published research. -SCBT QC
Date published: 2023-09-14
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IL-14 Antibody (16C5) is rated 4.0 out of 5 by 2.
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