IL-13Rα2 Double Nickase Plasmid (h): sc-402525-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IL-13Rα2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IL-13Rα2 Double Nickase Plasmid (h) and IL-13Rα2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IL13RA2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IL-13Rα2 Antibody (2K8): sc-134363
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-13Rα2 Double Nickase Plasmid (h) | sc-402525-NIC | 20 µg | $410.00 | |||
IL-13Rα2 Double Nickase Plasmid (h2) | sc-402525-NIC-2 | 20 µg | $410.00 |
Human IL13RA2 encodes IL-13Rα2, a high-affinity IL-13 binding chain that modulates type 2 cytokine signaling and can shape downstream JAK/STAT6-associated transcriptional programs by acting as a regulatory receptor component. Through control of IL-13 availability and receptor complex dynamics, IL-13Rα2 influences epithelial and stromal cell responses linked to inflammation, tissue remodeling, and extracellular matrix regulation. Altered IL13RA2 expression has been reported in contexts of chronic inflammatory microenvironments and in multiple tumor types where IL-13–responsive pathways affect cell migration, invasion, and immune interactions. These features make IL13RA2 a useful target for dissecting cytokine receptor biology, receptor trafficking, and microenvironment-driven signaling phenotypes in human cell models.
IL-13Rα2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IL13RA2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IL13RA2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IL13RA2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IL13RA2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.