IL-1 beta/IL1B Double Nickase Plasmid (m): sc-421097-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IL-1 beta/IL1B Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IL-1 beta/IL1B Double Nickase Plasmid (m) and IL-1 beta/IL1B Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Il1b. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IL-1 beta/IL1B Antibody (B122): sc-12742
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-1 beta/IL1B Double Nickase Plasmid (m) | sc-421097-NIC | 20 µg | $410.00 | |||
IL-1 beta/IL1B Double Nickase Plasmid (m2) | sc-421097-NIC-2 | 20 µg | $410.00 |
Mouse Il1b encodes interleukin‑1 beta (IL‑1β), a potent pro‑inflammatory cytokine produced primarily by activated myeloid cells and processed into its mature form following inflammasome‑dependent caspase‑1 cleavage. IL‑1β signaling through IL‑1 receptor complexes drives NF‑κB and MAPK pathway activation, promoting transcription of chemokines, adhesion molecules, and additional cytokines that coordinate leukocyte recruitment and innate immune amplification. This axis is tightly linked to pyroptosis, fever responses, and crosstalk with TNF and IL‑6 networks, shaping tissue inflammation and remodeling. Dysregulated IL‑1β production is widely used as a molecular readout in mouse models of infection, autoimmune and autoinflammatory syndromes, metabolic inflammation, and neuroinflammatory processes.
IL-1 beta/IL1B Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Il1b locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Il1b. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Il1b function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Il1b-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.