IL-1 beta/IL1B Double Nickase Plasmid (h): sc-400078-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IL-1 beta/IL1B Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IL-1 beta/IL1B Double Nickase Plasmid (h) and IL-1 beta/IL1B Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IL1B. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IL-1 beta/IL1B Antibody (B122): sc-12742
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-1 beta/IL1B Double Nickase Plasmid (h) | sc-400078-NIC | 20 µg | $410.00 | |||
IL-1 beta/IL1B Double Nickase Plasmid (h2) | sc-400078-NIC-2 | 20 µg | $410.00 |
Human IL1B encodes interleukin-1 beta (IL-1β), a potent pro-inflammatory cytokine produced as an inactive precursor that is proteolytically matured downstream of inflammasome activation and caspase-1 activity. IL-1β signaling through IL-1R1 and accessory proteins triggers MyD88-dependent cascades, including NF-κB and MAPK pathways, to induce secondary cytokines, chemokines, and adhesion molecules that coordinate innate immune responses. IL1B is tightly linked to inflammatory cell recruitment, fever response, and tissue remodeling, and dysregulated expression is associated with chronic inflammatory and autoimmune pathobiology. As a central node in cytokine networks, IL1B is widely studied in macrophage biology, inflammasome signaling, and models of sterile inflammation and infection-driven immune activation.
IL-1 beta/IL1B Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IL1B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IL1B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IL1B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IL1B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.