Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Ikaros Double Nickase Plasmid (h): sc-400530-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ikaros Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Ikaros Double Nickase Plasmid (h) and Ikaros Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IKZF1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Ikaros Antibody (E-2): sc-398265
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ikaros Double Nickase Plasmid (h) | sc-400530-NIC | 20 µg | $410.00 | |||
Ikaros Double Nickase Plasmid (h2) | sc-400530-NIC-2 | 20 µg | $410.00 |
IKZF1 encodes Ikaros, a zinc-finger transcription factor that orchestrates hematopoietic lineage specification and immune cell differentiation by regulating chromatin accessibility and transcriptional programs. Ikaros integrates signaling inputs to control gene networks involved in lymphoid development, B cell receptor signaling competence, cytokine-responsive transcription, and cell-cycle checkpoints. Through recruitment of chromatin remodeling and histone-modifying complexes, it influences enhancer and promoter activity across developmental stages. Dysregulation of IKZF1-dependent transcription is associated with altered lymphocyte maturation and is frequently studied in the context of hematologic disease mechanisms and leukemogenic transcriptional rewiring.
Ikaros Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IKZF1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IKZF1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IKZF1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IKZF1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.