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western blotting image from recommended NFKBIA/IkB alpha (H-4) monoclonal antibody replacement. Image 354367 — Questo anticorpo policlonale è stato sospeso. Consulta il nostro anticorpo monoclonale NFKBIA/IkB alpha (H-4) raccomandato (sono riportati i dati del campione).

Questo anticorpo policlonale è stato sospeso. Consulta il nostro anticorpo monoclonale NFKBIA/IkB alpha (H-4) raccomandato (sono riportati i dati del campione).

Anticorpo IκB-α (C-21): sc-371

4.8(30)

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I kappa B- alpha Antibody C-21 è un policlonale di coniglio IgG fornito in 100 µg/ml; o come IgGdi capra fornito in , sc-371-G
epitopo mappato al C-terminus di IκB-α di origine human
Anticorpo policlonale fuori produzione

Vedere citazioni di prodotto (907)

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Descrizione

VEDI ANCHE...

Anticorpi addizionali I κ B inclusi I kappa B-α, I kappa B-β e I κ B-ε

Solo per ricerca in vitro. Non destinato ad uso Diagnostico o Terapeutico.

Alexa Fluor^® è un marchio registrato di Molecular Probes Inc., OR., USA

Informazioni ordini

NFKBIA/IkB alpha (H-4): sc-1643 [ Monoclonale raccomandato come sostituzione per IκB-α (C-21) ]

Nome del prodotto	Codice del prodotto	UNITÀ	Prezzo	Quantità	Preferiti
NFKBIA/IkB alpha Anticorpo (H-4)	sc-1643	200 µg/ml	$316.00
NFKBIA/IkB alpha (H-4): m-IgG Fc BP-HRP Bundle	sc-528129	200 µg Ab; 10 µg BP	$354.00
NFKBIA/IkB alpha (H-4): m-IgGκ BP-HRP Bundle	sc-520432	200 µg Ab, 40 µg BP	$354.00
NFKBIA/IkB alpha Anticorpo (H-4) AC	sc-1643 AC	500 µg/ml, 25% agarose	$416.00
NFKBIA/IkB alpha Anticorpo (H-4) HRP	sc-1643 HRP	200 µg/ml	$316.00
NFKBIA/IkB alpha Anticorpo (H-4) FITC	sc-1643 FITC	200 µg/ml	$330.00
NFKBIA/IkB alpha Anticorpo (H-4) PE	sc-1643 PE	200 µg/ml	$343.00
NFKBIA/IkB alpha Anticorpo (H-4) Alexa Fluor^® 488	sc-1643 AF488	200 µg/ml	$357.00
NFKBIA/IkB alpha Anticorpo (H-4) Alexa Fluor^® 546	sc-1643 AF546	200 µg/ml	$357.00
NFKBIA/IkB alpha Anticorpo (H-4) Alexa Fluor^® 594	sc-1643 AF594	200 µg/ml	$357.00
NFKBIA/IkB alpha Anticorpo (H-4) Alexa Fluor^® 647	sc-1643 AF647	200 µg/ml	$357.00
NFKBIA/IkB alpha Anticorpo (H-4) Alexa Fluor^® 680	sc-1643 AF680	200 µg/ml	$357.00
NFKBIA/IkB alpha Anticorpo (H-4) Alexa Fluor^® 790	sc-1643 AF790	200 µg/ml	$357.00
NFKBIA/IkB alpha Anticorpo (H-4) TRITC	sc-1643 TRITC	200 µg/ml	$320.00

Hi, I've tried this antibody in WB using 30 ug of rat  liver cytoplasm lysate. I blocked nitrocellulose membrane with 10% skim milk for 1 h followed by overnight incubation with 1:100 primary antibody. Unfortunately I saw only a band at 90kDa.

Richiesta da: dany58

Thanks for your feedback. We are sorry to hear that this antibody did not perform for you as expected. We want you to be successful in your research. Please contact our Technical Service Department by phone, e-mail or live chat. We are happy to help you troubleshoot the antibody. Santa Cruz Biotechnology does warranty our antibodies for one year from the date of purchase. If we are unable to successfully troubleshoot your application we will send you a replacement antibody free of charge. Please contact our Technical Service by phone or email or by Live Chat if you need support or additional information

Risposta da: SCBT Heidelberg Support

Data di pubblicazione: 2018-11-27

hi, i keep getting two bands using this antibody. one band appears at around 37 kda and another one above 37 kda. could you explain what i can do to solve this problem? i'm using hacat (human keratinocyte cell line), 5% skim milk for blocking thank you

Richiesta da: shiba

Thank you for your question about our rabbit polyclonal antibody, sc-371: IkB-alpha (C-21). The expected molecular weight for IkB-alpha is approximately 37 kDa. This protein may also be phosphorylated which is one possible explanation for why you may also be detecting an additional slightly higher molecular weight band in your samples. I would recommend including additional controls in your experiment to confirm if the additional band above 37 kDa is also IkB-alpha, and a result of the binding of the specific primary antibody. One suggestion is to treat your lysates with Lambda Phosphatase: sc-200312 (https://www.scbt.com/scbt/product/lambda-phosphatase?requestFrom=search) to release phosphate groups and observe the difference in detection by Western blot. I would also recommend to perform a secondary antibody control blot (without primary antibody) to confirm that there is not any non-specific binding of your secondary antibody alone. Please contact our Technical Service Department for additional support in troubleshooting this antibody in your research application. https://www.scbt.com/scbt/resources/technical-service

Risposta da: Technical Support

Data di pubblicazione: 2018-12-18

Rated 5 di 5 di EBV girl da Good for western blotFor western blot, use at 1:1000 in 5% milk TBST, overnight incubation with rocking at 4 degrees C. Example of published result: PMID 29950426

Data di pubblicazione: 2020-01-09

Rated 5 di 5 di NatasaV da IkB antibody in rat samplesIt is working very well in the rat samples without unspecific bands

Data di pubblicazione: 2019-08-02

Rated 5 di 5 di sun2019 da quality of anti-IkBa antibodyThis antibody's quality is very good, and works very well in our hand.

Data di pubblicazione: 2019-01-15

Rated 4 di 5 di KuanYH da Nice primary antibodyThe primary antibody is available for Western blotting in mice sample.

Data di pubblicazione: 2017-06-13

Rated 5 di 5 di Eshu da Works really wellI have used this antibody for nearly five years and it worked really well with bone-marrow derived dendritic cell extracts of BALB/c and C57BL/6 mice. There were no non-specific bands and the band intensity for the specific bands was also very good.

Data di pubblicazione: 2017-06-06

Rated 4 di 5 di Steventian da Excellent experience.I bought this antobody for my current research project involved in the IkB-alpha protein detection by western blot assay for the mice kidney samples, this antibody works very well!

Data di pubblicazione: 2017-02-12

Rated 5 di 5 di Carme da Very clean immunoblots and high sensitivityThis is a great antibody for immunoblot analisis of endogenous IkBalpha. Very clean western blots and it can be diluted even 1:5000.

Data di pubblicazione: 2016-12-21

Rated 5 di 5 di Jing H da Great result in WBGreat result in WB using human and rat lungs tissue.

Data di pubblicazione: 2016-11-04

Anticorpo IκB-α (C-21) Recensioni - pagina 2

Anticorpo IκB-α (C-21) valutazione 4.8 di 5 di 30.

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Anticorpo IκB-α (C-21): sc-371

NFKBIA/IkB alpha Anticorpo (H-4)

NFKBIA/IkB alpha (H-4): m-IgG Fc BP-HRP Bundle

NFKBIA/IkB alpha (H-4): m-IgGκ BP-HRP Bundle

NFKBIA/IkB alpha Anticorpo (H-4) AC

NFKBIA/IkB alpha Anticorpo (H-4) HRP

NFKBIA/IkB alpha Anticorpo (H-4) FITC

NFKBIA/IkB alpha Anticorpo (H-4) PE

NFKBIA/IkB alpha Anticorpo (H-4) Alexa Fluor® 488

NFKBIA/IkB alpha Anticorpo (H-4) Alexa Fluor® 546

NFKBIA/IkB alpha Anticorpo (H-4) Alexa Fluor® 594

NFKBIA/IkB alpha Anticorpo (H-4) Alexa Fluor® 647

NFKBIA/IkB alpha Anticorpo (H-4) Alexa Fluor® 680

NFKBIA/IkB alpha Anticorpo (H-4) Alexa Fluor® 790

NFKBIA/IkB alpha Anticorpo (H-4) TRITC

Hi, I've tried this antibody in WB using 30 ug of rat &nbsp;liver cytoplasm lysate. I blocked nitrocellulose membrane with 10% skim milk for 1 h followed by overnight incubation with 1:100 primary antibody. Unfortunately I saw only a band at 90kDa.

hi, i keep getting two bands using this antibody. one band appears at around 37 kda and another one above 37 kda. could you explain what i can do to solve this problem? i'm using hacat (human keratinocyte cell line), 5% skim milk for blocking thank you

NFKBIA/IkB alpha Anticorpo (H-4) Alexa Fluor^® 488

NFKBIA/IkB alpha Anticorpo (H-4) Alexa Fluor^® 546

NFKBIA/IkB alpha Anticorpo (H-4) Alexa Fluor^® 594

NFKBIA/IkB alpha Anticorpo (H-4) Alexa Fluor^® 647

NFKBIA/IkB alpha Anticorpo (H-4) Alexa Fluor^® 680

NFKBIA/IkB alpha Anticorpo (H-4) Alexa Fluor^® 790

Hi, I've tried this antibody in WB using 30 ug of rat liver cytoplasm lysate. I blocked nitrocellulose membrane with 10% skim milk for 1 h followed by overnight incubation with 1:100 primary antibody. Unfortunately I saw only a band at 90kDa.