IKAP Antibody (H-11): sc-376509
- IKAP Antibody (H-11) is a mouse monoclonal IgG1 κ IKAP antibody provided at 200 µg/ml
- raised against amino acids 1031-1332 of IKAP of human origin
- IKAP Antibody (H-11) is recommended for detection of IKAP of human origin by WB, IP, IF and ELISA
- Anti-IKAP Antibody (H-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for IKAP Antibody (H-11) for WB applications. These reagents are now offered in bundles with IKAP Antibody (H-11) (see ordering information below).
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IKAP Antibody (H-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects IKAP protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). IKAP (H-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. IKAP protein, also known as IKK-complex-associated protein, plays a crucial role in the assembly and activation of the IKK complex, which is essential for the phosphorylation and subsequent degradation of the inhibitory protein IκB. This process is vital for the activation of the transcription factor NFκB, which regulates numerous genes involved in immune response, inflammation, and cell survival. IKAP interaction with the IKK complex, particularly with IKKα and IKKβ, facilitates the formation of an active kinase complex necessary for the phosphorylation of IκB on specific serine residues. This phosphorylation event is a key regulatory step in the NFκB signaling pathway, allowing NFκB to translocate to the nucleus and initiate the transcription of target genes. IKAP function as a scaffold protein underscores its importance in maintaining the integrity and functionality of the IKK complex, making anti-IKAP antibody (H-11) a valuable tool for studying NFκB signaling and its implications in various diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
IKAP Antibody (H-11) References:
- Rel/NF-kappa B/I kappa B family: intimate tales of association and dissociation. | Verma, IM., et al. 1995. Genes Dev. 9: 2723-35. PMID: 7590248
- NF-kappa B: a lesson in family values. | Thanos, D. and Maniatis, T. 1995. Cell. 80: 529-32. PMID: 7867060
- CHUK, a new member of the helix-loop-helix and leucine zipper families of interacting proteins, contains a serine-threonine kinase catalytic domain. | Connelly, MA. and Marcu, KB. 1995. Cell Mol Biol Res. 41: 537-49. PMID: 8777433
- MAP3K-related kinase involved in NF-kappaB induction by TNF, CD95 and IL-1. | Malinin, NL., et al. 1997. Nature. 385: 540-4. PMID: 9020361
- Identification and characterization of an IkappaB kinase. | Régnier, CH., et al. 1997. Cell. 90: 373-83. PMID: 9244310
- A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB. | DiDonato, JA., et al. 1997. Nature. 388: 548-54. PMID: 9252186
- Signal transduction. Docking IkappaB kinases. | Scheidereit, C. 1998. Nature. 395: 225-6. PMID: 9751044
- IKAP is a scaffold protein of the IkappaB kinase complex. | Cohen, L., et al. 1998. Nature. 395: 292-6. PMID: 9751059
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IKAP Antibody (H-11) | sc-376509 | 200 µg/ml | $322.00 | |||
IKAP Antibody (H-11): m-IgG Fc BP-HRP Bundle | sc-529889 | 200 µg Ab; 10 µg BP | $361.00 | |||
IKAP Antibody (H-11): m-IgGκ BP-HRP Bundle | sc-522983 | 200 µg Ab, 40 µg BP | $361.00 | |||
IKAP Antibody (H-11): m-IgG1 BP-HRP Bundle | sc-543746 | 200 µg Ab; 20 µg BP | $361.00 | |||
IKAP Antibody (H-11) AC | sc-376509 AC | 500 µg/ml, 25% agarose | $424.00 | |||
IKAP Antibody (H-11) HRP | sc-376509 HRP | 200 µg/ml | $322.00 | |||
IKAP Antibody (H-11) FITC | sc-376509 FITC | 200 µg/ml | $336.00 | |||
IKAP Antibody (H-11) PE | sc-376509 PE | 200 µg/ml | $349.00 | |||
IKAP Antibody (H-11) Alexa Fluor® 488 | sc-376509 AF488 | 200 µg/ml | $364.00 | |||
IKAP Antibody (H-11) Alexa Fluor® 546 | sc-376509 AF546 | 200 µg/ml | $364.00 | |||
IKAP Antibody (H-11) Alexa Fluor® 594 | sc-376509 AF594 | 200 µg/ml | $364.00 | |||
IKAP Antibody (H-11) Alexa Fluor® 647 | sc-376509 AF647 | 200 µg/ml | $364.00 | |||
IKAP Antibody (H-11) Alexa Fluor® 680 | sc-376509 AF680 | 200 µg/ml | $364.00 | |||
IKAP Antibody (H-11) Alexa Fluor® 790 | sc-376509 AF790 | 200 µg/ml | $364.00 |