IGSF22 Double Nickase Plasmid (h): sc-415302-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IGSF22 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IGSF22 Double Nickase Plasmid (h) and IGSF22 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IGSF22. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IGSF22 Double Nickase Plasmid (h) | sc-415302-NIC | 20 µg | $410.00 | |||
IGSF22 Double Nickase Plasmid (h2) | sc-415302-NIC-2 | 20 µg | $410.00 |
IGSF22 (immunoglobulin superfamily member 22) encodes a single-pass membrane protein with extracellular Ig-like domains, consistent with roles in cell–cell recognition and adhesion. Expression patterns reported for IGSF family members suggest involvement in tissue-specific communication programs that influence cytoskeletal organization, neurite outgrowth, and synapse-associated remodeling. By modulating membrane-proximal signaling and contact-dependent interactions, IGSF22 may intersect with pathways controlling cellular differentiation and connectivity. Dysregulated adhesion and surface receptor networks are frequently implicated in neurodevelopmental and oncogenic processes, making IGSF22 a relevant target for mechanistic studies of cell interaction phenotypes.
IGSF22 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IGSF22 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IGSF22. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IGSF22 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IGSF22-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.