IgG λ light chain Antibody (7G12): sc-69915

IgG λ light chain Antibody (7G12) is a rat monoclonal IgG2a kappa light chain antibody that detects IgG lambda light chain protein of mouse origin by enzyme-linked immunosorbent assay (ELISA). Anti-IgG lambda light chain antibody (7G12) is available as the non-conjugated format. IgG lambda light chain plays a crucial role in immune response, as one of two types of light chains that pair with heavy chains to form functional antibodies. Lambda light chain contributes to recognizing and neutralizing pathogens, supporting body′s defense mechanisms. Lambda light chain influences specificity and affinity of antibodies for target antigens. Understanding lambda light chain structure and function aids therapeutic antibody development and diagnostic applications, as light chain expression variations can indicate various diseases, including certain cancers. Basic structural unit of most mammalian antibodies consists of four polypeptide chains—two light chains and two heavy chains—connected by disulfide bonds. Each light chain includes variable and constant domains, with variable region genes assembled from V, C, and J genes. Human lambda light chain genes map to chromosome 22, and single light chain type expression in individual B cells remains critical for antibody diversity and specificity.

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IgG λ light chain Antibody (7G12) References:

1. The mysterious immunoglobulin light chain.  |  Pilström, L. 2002. Dev Comp Immunol. 26: 207-15. PMID: 11696386
2. Expression of immunoglobulin kappa light chain constant region in abnormal human cervical epithelial cells.  |  Li, M., et al. 2004. Int J Biochem Cell Biol. 36: 2250-7. PMID: 15313470
3. Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen.  |  Horejsí, V., et al. 1986. Folia Biol (Praha). 32: 12-25. PMID: 2422063
4. Evolution of human immunoglobulin kappa J region genes.  |  Hieter, PA., et al. 1982. J Biol Chem. 257: 1516-22. PMID: 6276389
5. Novel kappa light-chain gene rearrangements in mouse lambda light chain-producing B lymphocytes.  |  Durdik, J., et al. Nature. 307: 749-52. PMID: 6422305
6. The rat mixed lymphocyte reaction: roles of a dendritic cell in intestinal lymph and T-cell subsets defined by monoclonal antibodies.  |  Mason, DW., et al. 1981. Immunology. 44: 75-87. PMID: 6456222
7. Cloned human and mouse kappa immunoglobulin constant and J region genes conserve homology in functional segments.  |  Hieter, PA., et al. 1980. Cell. 22: 197-207. PMID: 6775818
8. Committed T lymphocyte stem cells of rats. Characterization by surface W3/13 antigen and radiosensitivity.  |  Dyer, MJ. and Hunt, SV. 1981. J Exp Med. 154: 1164-77. PMID: 6974759