IgA₁ (h2): 293T Lysate: sc-114781

IgA1 (h2): 293T Lysate, derived from the HEK 293T cell line, plays a vital role in the research of human Immunoglobulin A1 (IgA1), specifically within non-clinical scientific studies focusing on immunology and molecular biology. The 293T cells are a modified version of the original HEK 293 line, augmented to express the SV40 large T antigen, facilitating the high-level expression of episomal DNA. This enhancement is crucial for studies requiring robust protein expression, such as those involving IgA1, a major mucosal antibody. Researchers utilize this lysate to explore IgA1's unique structural and functional properties, including its role in mucosal immunity, its molecular assembly, and its secretion pathways. Techniques such as immunoprecipitation, Western blotting, and ELISA are employed to analyze IgA1's glycosylation patterns and interaction with other components of the immune system, like the polymeric immunoglobulin receptor (pIgR), which mediates its transport across epithelial cells. This lysate aids in understanding the fundamental aspects of antibody function and distribution, specifically how IgA1 contributes to the first line of defense at mucosal surfaces.