Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
IFITM2 Double Nickase Plasmid (h): sc-403889-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IFITM2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IFITM2 Double Nickase Plasmid (h) and IFITM2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IFITM2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IFITM2 Double Nickase Plasmid (h) | sc-403889-NIC | 20 µg | $410.00 | |||
IFITM2 Double Nickase Plasmid (h2) | sc-403889-NIC-2 | 20 µg | $410.00 |
IFITM2 (interferon induced transmembrane protein 2) is a type II transmembrane protein induced by type I and type II interferon signaling and functions as an intrinsic restriction factor that limits entry and early replication steps of diverse enveloped viruses. It localizes primarily to endosomal and plasma membrane compartments, where it alters membrane properties and trafficking to impede viral fusion and uptake, linking it to innate immune defense and interferon-stimulated gene networks. IFITM2 activity intersects with pathways governing endocytosis, vesicle organization, and inflammatory signaling, and its expression is frequently used as a readout of interferon pathway activation. Dysregulated IFITM2 expression has been studied in the context of aberrant immune activation and infection-associated inflammatory states, supporting its relevance for mechanistic studies of host–pathogen interactions.
IFITM2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IFITM2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IFITM2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IFITM2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IFITM2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.