ICAM-1/CD54 (h): 293T Lysate: sc-176625

ICAM-1 (h): 293T Lysate is derived from the HEK 293T cell line, specifically utilized for studying the Intercellular Adhesion Molecule 1 (ICAM-1) in human cells. This lysate plays a crucial role in examining ICAM-1's functions in cellular adhesion and signaling pathways related to immune responses, entirely within non-clinical research settings. The 293T cell line, a variant of the HEK 293 cells, is modified to express the SV40 large T antigen, promoting plasmid amplification and facilitating higher protein expression levels. This characteristic is particularly beneficial for studies involving transient transfection, enabling researchers to analyze the effects of gene manipulation on ICAM-1 expression. Utilizing this lysate, scientists conduct investigations on the molecular dynamics of cell-cell interactions, particularly how ICAM-1 mediates leukocyte endothelial transmigration. Through techniques like immunoprecipitation, Western blotting, and flow cytometry, research on this lysate provides insights into the signaling cascades that regulate immune cell trafficking and adhesion under various stress conditions. These studies contribute to a broader understanding of the molecular mechanisms governing cellular communication and immune surveillance.