ICAM-1 Antibody (28) recommended for detection of ICAM-1 of human origin by WB and IP

ICAM-1 Antibody (28): sc-135946

ICAM-1 Antibody (28) is rated 4.5 out of 5 by 2.
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Datasheets

    • ICAM-1 Antibody (28) is a mouse monoclonal IgG1 provided at 50 µg/0.5 ml
    • raised against amino acids 46-160 of ICAM-1 of human origin
    • recommended for detection of ICAM-1 of human origin by WB and IP
    • See ICAM-1 (G-5): sc-8439 for ICAM-1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

I am using ICAM-1 (28): sc-135946 antibody for immunoprecipitation, how much antibody should I use?

Asked by: jerojero
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from bold band in Jurkat and TNF treated U bold band in Jurkat and TNF treated U-937 whole cell lysates. -SCBT QC
Date published: 2015-04-24
Rated 4 out of 5 by from Wide band obtained in HCT Wide band obtained in HCT-8 whole cell lysate. -SCBT QC
Date published: 2013-10-23
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