Hygromycin B solution (CAS 31282-04-9)

Usage :

• This product is provided as 20 mL of a sterile filtered solution in PBS.


• Appearance: Solution color can vary from light yellow to a dark brown or caramel color. Concentrated Hygromycin solution tends to have a darker appearance.


• The working concentration for the purpose of selection varies with cell type, media, growth conditions and cell metabolic rate. A titration experiment for each cell type should be performed to determine the amount of Hygromycin B needed to kill non-transfected cells.


• Recommended concentrations for the selection of resistant cells is 25-1000 μg/ml. Commonly used concentrations for selection are 200 μg/ml for mammalian cells, 20-200 μg/ml for plant cells & bacteria cells and 200-1000 μg/ml for fungi. Your optimum concentration should be tested experimentally.


• Control cells should die within 5-7 days after addition of the antibiotic allowing colonies of resistant cells to form by 10–14 days.

Formulation :

50 mg/ml in PBS

Physical State :

Liquid

Derived From :

Streptomyces hygroscopicus

Solubility :

Soluble in water, methanol, buffer solution, and ethanol.

Storage :

Store at 4° C

RTECS :

WK2130000

PubChem CID :

Merck Index :

14: 4852

MDL Number :

MFCD06795479

EC Number :

250-545-5

SMILES :

CN[C@H]1C[C@H]([C@@H]([C@H]([C@@H]1O)O[C@H]2[C@@H]3[C@H]([C@H]([C@H](O2)CO)O)OC4(O3)[C@@H]([C@@H]([C@H]([C@H](O4)[C@H](CO)N)O)O)O)O)N