Date published: 2025-11-2

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Hydroxynaphthol blue (CAS 63451-35-4)

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Alternate Names:
1-(2-Hydroxy-4-sulfo-1-naphthylazo)-2-naphthol-3
CAS Number:
63451-35-4
Molecular Weight:
620.47
Molecular Formula:
C20H11N2Na3O11S3
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Hydroxynaphthol blue, a chemical dye employed in scientific research, serves diverse applications within the field. This water-soluble dye finds utility in staining, imaging, and biochemical studies. For over a century, it has been utilized as a staining agent for cells and tissues, allowing researchers to delve into the intricacies of cell structure and function. In addition, hydroxynaphthol blue serves as useful in biochemical studies, enabling the staining of nucleic acids, proteins, lipids, and carbohydrates. Moreover, it facilitates the visualization and quantitative assessment of molecular presence within cells and tissues. Further applications include immunohistochemistry, flow cytometry, and microscopy.


Hydroxynaphthol blue (CAS 63451-35-4) References

  1. Visual detection of pandemic influenza A H1N1 Virus 2009 by reverse-transcription loop-mediated isothermal amplification with hydroxynaphthol blue dye.  |  Ma, XJ., et al. 2010. J Virol Methods. 167: 214-7. PMID: 20381535
  2. Visual detection of turkey coronavirus RNA in tissues and feces by reverse-transcription loop-mediated isothermal amplification (RT-LAMP) with hydroxynaphthol blue dye.  |  Cardoso, TC., et al. 2010. Mol Cell Probes. 24: 415-7. PMID: 20732411
  3. Visual detection of human infection with influenza A (H7N9) virus by subtype-specific reverse transcription loop-mediated isothermal amplification with hydroxynaphthol blue dye.  |  Nie, K., et al. 2013. Clin Microbiol Infect. 19: E372-5. PMID: 23718218
  4. Rapid and sensitive detection of human astrovirus in water samples by loop-mediated isothermal amplification with hydroxynaphthol blue dye.  |  Yang, BY., et al. 2014. BMC Microbiol. 14: 38. PMID: 24524254
  5. Visual detection of norovirus genogroup II by reverse transcription loop-mediated isothermal amplification with hydroxynaphthol blue dye.  |  Luo, J., et al. 2014. Food Environ Virol. 6: 196-201. PMID: 24752892
  6. Rapid and visual Chlamydia trachomatis detection using loop-mediated isothermal amplification and hydroxynaphthol blue.  |  Choopara, I., et al. 2017. Lett Appl Microbiol. 64: 51-56. PMID: 27689353
  7. Rapid and simple identification of carbapenemase genes, bla NDM, bla OXA-48, bla VIM, bla IMP-14 and bla KPC groups, in Gram-negative bacilli by in-house loop-mediated isothermal amplification with hydroxynaphthol blue dye.  |  Srisrattakarn, A., et al. 2017. World J Microbiol Biotechnol. 33: 130. PMID: 28585170
  8. Detection and quantification of transition metal leaching in metal affinity chromatography with hydroxynaphthol blue.  |  Kokhan, O. and Marzolf, DR. 2019. Anal Biochem. 582: 113347. PMID: 31251926
  9. Point-of-Care Chlamydia trachomatis Detection Using Loop-Mediated Isothermal Amplification and Hydroxynaphthol Blue.  |  Somboonna, N. and Choopara, I. 2019. Methods Mol Biol. 2042: 11-17. PMID: 31385266
  10. Rapid visual detection of Vibrio parahaemolyticus in seafood samples by loop-mediated isothermal amplification with hydroxynaphthol blue dye.  |  Anupama, KP., et al. 2020. World J Microbiol Biotechnol. 36: 76. PMID: 32390085
  11. Specific and sensitive, ready-to-use universal fungi detection by visual color using ITS1 loop-mediated isothermal amplification combined hydroxynaphthol blue.  |  Choopara, I., et al. 2021. PeerJ. 9: e11082. PMID: 33777539
  12. Rapid visual detection of Aeromonas salmonicida by loop-mediated isothermal amplification with hydroxynaphthol blue dye.  |  Zhou, S., et al. 2021. J Fish Dis. 44: 1993-2001. PMID: 34411329
  13. A simple colorimetric detection of porcine epidemic diarrhea virus by reverse transcription loop-mediated isothermal amplification assay using hydroxynaphthol blue metal indicator.  |  Kim, JK., et al. 2021. J Virol Methods. 298: 114289. PMID: 34536488
  14. Closed-tube saltatory rolling circle amplification with hydroxynaphthol blue for visual on-site detection of peanut as an allergenic food.  |  Yang, Q., et al. 2022. Food Chem. 393: 133408. PMID: 35691067
  15. An optimized visual loop mediated isothermal amplification assay for efficient detection of minute virus of mice with hydroxynaphthol blue dye.  |  Zhang, X., et al. 2022. J Virol Methods. 308: 114575. PMID: 35798197

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Hydroxynaphthol blue, 10 g

sc-235341
10 g
$88.00