HXK II Antibody (1A7): sc-130358

HXK II Antibody (1A7) is a mouse monoclonal IgG1 kappa light chain antibody that detects HXK II in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-HXK II antibody (1A7) is available in a non-conjugated form, allowing for versatile experimental use. HXK II plays a crucial role in glucose metabolism by catalyzing the phosphorylation of glucose to glucose-6-phosphate, the first step in the glycolytic pathway, which is essential for energy production in cells. This enzyme is particularly important in tissues that require high levels of glucose, such as adipose and skeletal muscle, where HXK II facilitates glucose uptake and utilization. The regulation of HXK II activity is vital for maintaining glucose homeostasis, and disruption can lead to metabolic disorders, including diabetes. Additionally, HXK II is one of four hexokinase isoenzymes, each with distinct regulatory properties and tissue distributions, highlighting the complexity of glucose metabolism in different physiological contexts. Understanding HXK II function and regulation is critical for developing therapeutic strategies aimed at metabolic diseases.

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HXK II Antibody (1A7) References:

1. HK2/hexokinase-II integrates glycolysis and autophagy to confer cellular protection.  |  Tan, VP. and Miyamoto, S. 2015. Autophagy. 11: 963-4. PMID: 26075878
2. Hexokinase-2 depletion inhibits glycolysis and induces oxidative phosphorylation in hepatocellular carcinoma and sensitizes to metformin.  |  DeWaal, D., et al. 2018. Nat Commun. 9: 446. PMID: 29386513
3. SUMOylation controls the binding of hexokinase 2 to mitochondria and protects against prostate cancer tumorigenesis.  |  Shangguan, X., et al. 2021. Nat Commun. 12: 1812. PMID: 33753739
4. Hexokinase 2 in Cancer: A Prima Donna Playing Multiple Characters.  |  Ciscato, F., et al. 2021. Int J Mol Sci. 22: PMID: 33946854
5. Hexokinase 2-driven glycolysis in pericytes activates their contractility leading to tumor blood vessel abnormalities.  |  Meng, YM., et al. 2021. Nat Commun. 12: 6011. PMID: 34650057
6. Microglial hexokinase 2 deficiency increases ATP generation through lipid metabolism leading to β-amyloid clearance.  |  Leng, L., et al. 2022. Nat Metab. 4: 1287-1305. PMID: 36203054
7. Dual roles of hexokinase 2 in shaping microglial function by gating glycolytic flux and mitochondrial activity.  |  Hu, Y., et al. 2022. Nat Metab. 4: 1756-1774. PMID: 36536134
8. Mitochondrial control of microglial phagocytosis by the translocator protein and hexokinase 2 in Alzheimer's disease.  |  Fairley, LH., et al. 2023. Proc Natl Acad Sci U S A. 120: e2209177120. PMID: 36787364
9. Hexokinase 2-mediated gene expression via histone lactylation is required for hepatic stellate cell activation and liver fibrosis.  |  Rho, H., et al. 2023. Cell Metab. 35: 1406-1423.e8. PMID: 37463576
10. Hexokinase 2 senses fructose in tumor-associated macrophages to promote colorectal cancer growth.  |  Yan, H., et al. 2024. Cell Metab. 36: 2449-2467.e6. PMID: 39471815

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HXK II Antibody (1A7) has 24 citations in a variety of scientific publications.

Primary antibodies like Anti-HXK II Antibody (1A7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HXK II Antibody (1A7) Specifications

Alternate Product Name: HXK II

Product Clone Name: 1A7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: HXK II

Epitope Species: human

Product Reactivity/Detection: HXK II

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 50 µg IgG₁ kappa light chain in 500 µl of PBS with < 0.1% sodium azide, 0.1% gelatin and 1% glycerol

Research Citations: 24 citations in various scientific publications

Available Conjugates/Forms for HXK II Antibody (1A7): N/A

The preferred HXK II antibody is HXK II Antibody (B-8): sc-374091.