Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Huntingtin Double Nickase Plasmid (m): sc-420825-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Huntingtin Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Huntingtin Double Nickase Plasmid (m) and Huntingtin Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Htt. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Huntingtin Antibody (3E10): sc-47757
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Huntingtin Double Nickase Plasmid (m) | sc-420825-NIC | 20 µg | $410.00 | |||
Huntingtin Double Nickase Plasmid (m2) | sc-420825-NIC-2 | 20 µg | $410.00 |
Mouse Htt encodes huntingtin, a large scaffold protein that supports neuronal homeostasis through roles in vesicular trafficking, endocytosis, and cytoskeletal dynamics. Huntingtin participates in transcriptional regulation and proteostasis networks, including autophagy and ubiquitin-dependent clearance, and influences synaptic function and intracellular transport. Perturbation of huntingtin levels or integrity impacts striatal and cortical neuron physiology and has been widely studied in the context of neurodegeneration and Huntington’s disease–related molecular phenotypes. In mouse systems, Htt provides a key entry point for dissecting pathway cross-talk between axonal transport, mitochondrial function, and stress-response signaling.
Huntingtin Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Htt locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Htt. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Htt function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Htt-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.