Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HUNK CRISPR/Cas9 KO Plasmid (h): sc-405786
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HUNK CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HUNK genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HUNK Antibody (B-7): sc-514689
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HUNK CRISPR/Cas9 KO Plasmid (h) | sc-405786 | 20 µg | $397.00 |
Overview
HUNK (hormonally upregulated Neu-associated kinase) encodes a serine/threonine protein kinase of the AMPK-related kinase family that integrates growth factor and hormone-responsive signaling with epithelial cell behavior. HUNK has been linked to regulation of cell survival programs, polarity maintenance, and vesicle/trafficking-associated processes that influence adhesion and migration, with reported connections to PI3K–AKT and MAPK network outputs in stress and proliferation contexts. Dysregulated HUNK expression or activity has been observed in multiple cancer-related models, where it is studied for roles in tumor cell adaptation, metastatic traits, and therapy response phenotypes. In addition, HUNK biology is relevant to kinase signaling cross-talk in breast and neural tissues, supporting mechanistic studies of pathway rewiring in disease-relevant cell states.
HUNK CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the HUNK gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HUNK together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HUNK open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HUNK protein expression.
This CRISPR knockout system enables efficient generation of HUNK-deficient cell models for investigation of HUNK signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting HUNK exon(s) critical for HUNK function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple HUNK genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HUNK CRISPR/Cas9 KO Plasmid (h) and HUNK CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HUNK locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HUNK HDR Plasmid (h) and HUNK HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HUNK homology arms to support homology-directed repair at defined HUNK target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.